Expression of Heterologous Cellulases inThermotogasp. Strain RQ2

Expression of Heterologous Cellulases in Thermotoga sp. Strain RQ2

The ability of Thermotoga spp. to degrade cellulose is limited due to a lack of exoglucanases. To address this deficiency, cellulase genes Csac_1076 (celA) and Csac_1078 (celB) from Caldicellulosiruptor saccharolyticus were cloned into T. sp. strain RQ2 for heterologous overexpression. Coding regions of Csac_1076 and Csac_1078 were fused to the signal peptide of TM1840 (amyA) and TM0070 (xynB),...

Heterologous Expression and Extracellular Secretion of Cellulases in Recombinant Microbes

Heterologous Expression of Bovine Prochymosin in Pichia pastoris GS115

Objectives: In present research we evaluate the expression of this critical enzyme in a eukaryotic system for future use in cheese industry. Materials and Methods: We have cloned bovine prochymosin gene in methylotrophic yeast, P. pastoris, using pPIC9K as an expression vector. The recombinant plasmid was transformed into the host by electroporation, and it was expre...

Heterologous Expression of Potato Virus Y Coat Protein, Isolate Pot187

Background: The advent of recombinant DNA technology has facilitated heterologous expression of proteins from various sources in different host systems including Escherichia coli. If a plant virus coat protein is expressed in the bacterium it can be used as the antigen for antibody preparation. Such a recombinant antigen preparation can be particularly useful where equipment such as ultracentri...

Exploitation of Aspergillus niger for the Heterologous Production of Cellulases and Hemicellulases

Filamentous fungi of the Aspergillus niger group are native soil saprophytic fungi. Industrial strains of this group have been extensively used for the production of plant degrading enzymes for the food and beverage, animal feed and paper-and-pulp industries. Recombinant DNA technology allows for the overproduction of these enzymes in copious amounts. The advantages and limitations of A. niger ...